UV‐inducible DNA exchange in hyperthermophilic archaea mediated by type IV pili

@article{Ajon2011UVinducibleDE,
  title={UV‐inducible DNA exchange in hyperthermophilic archaea mediated by type IV pili},
  author={Małgorzata Ajon and Sabrina Fr{\"o}ls and Marleen van Wolferen and Kilian Stoecker and Daniela Teichmann and Arnold J. M. Driessen and Dennis W. Grogan and Sonja-Verena Albers and Christa Schleper},
  journal={Molecular Microbiology},
  year={2011},
  volume={82}
}
Archaea, like bacteria and eukaryotes, contain proteins involved in various mechanisms of DNA repair, highlighting the importance of these processes for all forms of life. Species of the order Sulfolobales of hyperthermophilic crenarchaeota are equipped with a strongly UV‐inducible type IV pilus system that promotes cellular aggregation. Here we demonstrate by fluorescence in situ hybridization that cellular aggregates are formed based on a species‐specific recognition process and that UV… Expand

Paper Mentions

Swapping genes to survive – a new role for archaeal type IV pili
  • T. Allers
  • Biology, Medicine
  • Molecular microbiology
  • 2011
TLDR
This work has defined a new role for type IV pili in both the transfer of genes within species and the recovery from UV‐induced DNA damage. Expand
Molecular analysis of the UV-inducible pili operon from Sulfolobus acidocaldarius
TLDR
All genes present in the ups cluster are studied via gene deletion analysis with a focus on UpsX, a protein that shows no identifiable functional domains and UspX does not seem to be structurally essential for UV‐induced pili formation and cellular aggregation, but appears to be important for efficient DNA transfer. Expand
DNA Processing Proteins Involved in the UV-Induced Stress Response of Sulfolobales
TLDR
Next to the previously described role of UV-inducible pili in the exchange of DNA, this paper increases the knowledge of DNA transfer at the level of DNA processing and contributes to the overall understanding of the DNA exchange mechanism among Sulfolobales cells. Expand
Increase of positive supercoiling in a hyperthermophilic archaeon after UV irradiation
TLDR
This work shows that the hyperthermophilic archaeon Thermococcus nautili possesses a remarkable ability to recovery from extreme chromosomal damage, and suggests simple UV stress could be used to study archaeal DNA repair and responses to DSB. Expand
Species-Specific Recognition of Sulfolobales Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns
TLDR
Each Sulfolobus species seems to have developed a unique UpsA binding pocket and unique N-glycan composition to ensure aggregation and, consequently, also DNA exchange with cells from only the same species, which is essential for DNA repair by homologous recombination. Expand
Noncanonical Cell-to-Cell DNA Transfer in Thermus spp. Is Insensitive to Argonaute-Mediated Interference
TLDR
This work shows that T. thermophilus strains are capable of exchanging large DNA fragments by a novel mechanism that requires cell-to-cell contacts and employs components of the natural transformation machinery, thus allowing the organism to distinguish between DNA transferred from a mate and exogenous DNA acquired from unknown hosts. Expand
Haloferax Volcanii Strategies to Regulate Type IV Pilus Dependent Adhesion and Microcolony Formation
TLDR
Several mechanisms to regulate biofilm formation and dispersal in Hfx. Expand
Homologous recombination in the archaeon Sulfolobus acidocaldarius: effects of DNA substrates and mechanistic implications.
TLDR
Results indicate that HR events between gene-sized duplex DNAs and the S. acidocaldarius chromosome typically involve neither crossing over nor interference from a mismatch-activated anti-recombination system, as in the mechanism proposed for oligonucleotide-mediated transformation of Sulfolobus and other micro-organisms. Expand
A conserved hexanucleotide motif is important in UV-inducible promoters in Sulfolobus acidocaldarius
TLDR
A conserved, non-palindromic hexanucleotide motif upstream of the ups core promoter elements is identified to be essential for promoter activity. Expand
The archaeal Ced system imports DNA
TLDR
A set of genes directly involved in the transfer of chromosomal DNA between Sulfolobus acidocaldarius cells are described, and it is proposed that both Saci_0568 and saci_0748 are part of a previously unidentified DNA importer. Expand
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