UV‐inducible DNA exchange in hyperthermophilic archaea mediated by type IV pili

  title={UV‐inducible DNA exchange in hyperthermophilic archaea mediated by type IV pili},
  author={Małgorzata Ajon and Sabrina Fr{\"o}ls and Marleen van Wolferen and Kilian Stoecker and Daniela Teichmann and Arnold J. M. Driessen and Dennis W. Grogan and Sonja-Verena Albers and Christa Schleper},
  journal={Molecular Microbiology},
Archaea, like bacteria and eukaryotes, contain proteins involved in various mechanisms of DNA repair, highlighting the importance of these processes for all forms of life. Species of the order Sulfolobales of hyperthermophilic crenarchaeota are equipped with a strongly UV‐inducible type IV pilus system that promotes cellular aggregation. Here we demonstrate by fluorescence in situ hybridization that cellular aggregates are formed based on a species‐specific recognition process and that UV… 

Molecular analysis of the UV-inducible pili operon from Sulfolobus acidocaldarius

All genes present in the ups cluster are studied via gene deletion analysis with a focus on UpsX, a protein that shows no identifiable functional domains and UspX does not seem to be structurally essential for UV‐induced pili formation and cellular aggregation, but appears to be important for efficient DNA transfer.

DNA Processing Proteins Involved in the UV-Induced Stress Response of Sulfolobales

Next to the previously described role of UV-inducible pili in the exchange of DNA, this paper increases the knowledge of DNA transfer at the level of DNA processing and contributes to the overall understanding of the DNA exchange mechanism among Sulfolobales cells.

Increase of positive supercoiling in a hyperthermophilic archaeon after UV irradiation

This work shows that the hyperthermophilic archaeon Thermococcus nautili possesses a remarkable ability to recovery from extreme chromosomal damage, and suggests simple UV stress could be used to study archaeal DNA repair and responses to DSB.

Species-Specific Recognition of Sulfolobales Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns

Each Sulfolobus species seems to have developed a unique UpsA binding pocket and unique N-glycan composition to ensure aggregation and, consequently, also DNA exchange with cells from only the same species, which is essential for DNA repair by homologous recombination.

Noncanonical Cell-to-Cell DNA Transfer in Thermus spp. Is Insensitive to Argonaute-Mediated Interference

This work shows that T. thermophilus strains are capable of exchanging large DNA fragments by a novel mechanism that requires cell-to-cell contacts and employs components of the natural transformation machinery, thus allowing the organism to distinguish between DNA transferred from a mate and exogenous DNA acquired from unknown hosts.

Haloferax Volcanii Strategies to Regulate Type IV Pilus Dependent Adhesion and Microcolony Formation

Several mechanisms to regulate biofilm formation and dispersal in Hfx.

Homologous recombination in the archaeon Sulfolobus acidocaldarius: effects of DNA substrates and mechanistic implications.

Results indicate that HR events between gene-sized duplex DNAs and the S. acidocaldarius chromosome typically involve neither crossing over nor interference from a mismatch-activated anti-recombination system, as in the mechanism proposed for oligonucleotide-mediated transformation of Sulfolobus and other micro-organisms.

A conserved hexanucleotide motif is important in UV-inducible promoters in Sulfolobus acidocaldarius

A conserved, non-palindromic hexanucleotide motif upstream of the ups core promoter elements is identified to be essential for promoter activity.

The archaeal Ced system imports DNA

A set of genes directly involved in the transfer of chromosomal DNA between Sulfolobus acidocaldarius cells are described, and it is proposed that both Saci_0568 and saci_0748 are part of a previously unidentified DNA importer.

How hyperthermophiles adapt to change their lives: DNA exchange in extreme conditions

The different mechanisms of DNA transfer in hyperthermophiles are described, and how this may contribute to the survival and adaptation of hyperThermophilic archaea and bacteria to extreme environments.



Response of the Hyperthermophilic Archaeon Sulfolobus solfataricus to UV Damage

Strong transcriptional induction of genes which potentially encode functions for pilus formation suggested that conjugational activity might lead to enhanced exchange of genetic material and provided supporting evidence to a link between recombinational repair and conjugation events.

UV‐inducible cellular aggregation of the hyperthermophilic archaeon Sulfolobus solfataricus is mediated by pili formation

It is hypothesized that pili formation and subsequent cellular aggregation enhance DNA transfer among Sulfolobus cells to provide increased repair of damaged DNA via homologous recombination.

UV stimulation of chromosomal marker exchange in Sulfolobus acidocaldarius: implications for DNA repair, conjugation and homologous recombination at extremely high temperatures.

Results are interpreted as evidence that marker exchange is stimulated by recombinogenic DNA lesions formed as intermediates in the process of repairing UV photoproducts in the S. acidocaldarius chromosome.

Genetic responses of the thermophilic archaeon Sulfolobus acidocaldarius to short-wavelength UV light

These results provide the first evidence of error-prone DNA repair and genetic recombination induced by DNA damage in an archaeon from geothermal habitats.

Homologous recombination in Sulfolobus acidocaldarius: genetic assays and functional properties.

  • D. Grogan
  • Biology
    Biochemical Society transactions
  • 2009
Results indicate multiple discontinuous tracts in the majority of recombinants, suggesting that properties of the HR process could, in principle, account for many of the apparent peculiarities of intragenic recombination initiated by S. acidocaldarius conjugation.

Responses of hyperthermophilic crenarchaea to UV irradiation

A clear response to DNA damage was observed, with down- regulation of the DNA replication machinery, changes in transcriptional regulatory proteins, and up-regulation of the biosynthetic enzymes for beta-carotene, which has UV protective properties, and proteins that detoxify reactive oxygen species.

Sulfolobus Mutants, Generated via PCR Products, Which Lack Putative Enzymes of UV Photoproduct Repair

The phenotypic analyses of the disruptants confirmed that ORF Saci_1227 encodes a DNA photolyase which functions in vivo, but they could not implicate ORFSaci_1096 in repair of UV- or other externally induced DNA damage despite its similarity to genes encoding UV damage endonucleases.

High Spontaneous Mutation Rate in the Hyperthermophilic Archaeon Sulfolobus solfataricus Is Mediated by Transposable Elements

ABSTRACT We have isolated uracil-auxotrophic mutants of the hyperthermophilic archaeon Sulfolobus solfataricus in order to explore the genomic stability and mutational frequencies of this organism

Biological effects of DNA damage in the hyperthermophilic archaeon Sulfolobus acidocaldarius.

To investigate the generality of efficient double-strand break repair and damage-induced mutagenesis in hyperthermophilic archaea, we systematically measured the effects of five DNA-damaging agents