UPARSE: highly accurate OTU sequences from microbial amplicon reads

@article{Edgar2013UPARSEHA,
  title={UPARSE: highly accurate OTU sequences from microbial amplicon reads},
  author={Robert C. Edgar},
  journal={Nature Methods},
  year={2013},
  volume={10},
  pages={996-998}
}
Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with ≤1% incorrect bases in artificial microbial community tests, compared with >3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community. 
Highly Influential
This paper has highly influenced 254 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
Recent Discussions
This paper has been referenced on Twitter 158 times over the past 90 days. VIEW TWEETS

From This Paper

Topics from this paper.
1,373 Citations
2 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 1,373 extracted citations

References

Publications referenced by this paper.
Showing 1-2 of 2 references

np g © 2 01 3 N at ur e A m er ic a, In c. A ll rig

  • Sayers, E.W
  • Nucleic Acids Res. 40,
  • 2012

Similar Papers

Loading similar papers…