UPARSE: highly accurate OTU sequences from microbial amplicon reads

  title={UPARSE: highly accurate OTU sequences from microbial amplicon reads},
  author={Robert C. Edgar},
  journal={Nature Methods},
Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with ≤1% incorrect bases in artificial microbial community tests, compared with >3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community. 
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np g © 2 01 3 N at ur e A m er ic a, In c. A ll rig

  • Sayers, E.W
  • Nucleic Acids Res. 40,
  • 2012

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