UDPgalactose:ceramide galactosyltransferase of rat brain: a new method of purification and production of specific antibodies.

@article{Neskovic1986UDPgalactoseceramideGO,
  title={UDPgalactose:ceramide galactosyltransferase of rat brain: a new method of purification and production of specific antibodies.},
  author={Nenad M. Neskovic and Guy Roussel and J. L. Nussbaum},
  journal={Journal of neurochemistry},
  year={1986},
  volume={47 5},
  pages={1412-8}
}
A new method for purification of UDPgalactose:ceramide galactosyltransferase (EC 2.4.1.45) is described. The principal steps involved solvent extraction at -70 degrees C, Triton X-100 extraction, and DEAE-Sephadex and Blue Sepharose chromatography. The active configuration of the enzyme was stabilized by phospholipids and a rapid loss of enzymatic activity was observed after removal of these lipids. The inactive enzyme could be fully reactivated in the presence of brain phospholipids dispersed… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-4 of 4 extracted citations

Similar Papers

Loading similar papers…