UDP-glucuronate carboxy-lyase in cultured chondrocytes.

  title={UDP-glucuronate carboxy-lyase in cultured chondrocytes.},
  author={Kurian John and Nancy B. Schwartz and Helmut Ankel},
  journal={The Journal of biological chemistry},
  volume={252 19},
UDP-glucuronate carboxy-lyase has been demonstrated in chick chondrocytes in tissue culture. It occurs in the particulate fraction, and its activity is stimulated by exogenous NAD. The enzyme is allosterically activated by UDP-glucuronate and inhibited by UDP-xylose, n Values of 2.8 indicate positive cooperativity of at least three interacting sites on the enzyme. These data suggest that UDP-xylose concentration in chondrocytes is regulated by substrate activation and product inhibition of UDP… CONTINUE READING