UDP-glucosyltransferase activity toward exogenous substrates in Drosophila melanogaster.

  title={UDP-glucosyltransferase activity toward exogenous substrates in Drosophila melanogaster.},
  author={M. Real and J. Ferr{\'e} and F. J. Chapa},
  journal={Analytical biochemistry},
  volume={194 2},
  • M. Real, J. Ferré, F. J. Chapa
  • Published 1991
  • Biology, Medicine
  • Analytical biochemistry
  • To investigate the capacity of Drosophila extracts to glucosylate exogenous substrates we have developed a fast and sensitive method for the detection of UDP-glucosyltransferase activity using 4-nitrophenol, 1-naphthol, or 2-naphthol as substrates. High-performance liquid chromatography was used to separate and quantitate the reaction products, allowing detection of activities that produced as little as 1 pmol of 2-naphthol glucoside (fluorescence detection) or 16 pmol of 4-nitrophenol… CONTINUE READING
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