UDP-glucosamine as a substrate for dolichyl monophosphate glucosamine synthesis.

  title={UDP-glucosamine as a substrate for dolichyl monophosphate glucosamine synthesis.},
  author={William McDowell and Gisbert Weckbecker and Dietrich Keppler and Ralph T. Schwarz},
  journal={The Biochemical journal},
  volume={233 3},
The sugar nucleotide analogue UDP-glucosamine was found to function as a sugar donor in microsomal preparations of both chick-embryo cells and rat liver, yielding dolichyl monophosphate glucosamine (Dol-P-GlcN). This was characterized by t.l.c. and retention by DEAE-cellulose. Glucosamine was the only water-soluble product released on mild acid hydrolysis. Dol-P-GlcN did not serve as substrate by transferring its glucosamine moiety to dolichol-linked oligosaccharide. Competition experiments… 
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