UDP-N-acetylmuramic acid l-alanine ligase (MurC) inhibition in a tolC mutant Escherichia coli strain leads to cell death.

@article{Humnabadkar2014UDPNacetylmuramicAL,
  title={UDP-N-acetylmuramic acid l-alanine ligase (MurC) inhibition in a tolC mutant Escherichia coli strain leads to cell death.},
  author={Vaishali Humnabadkar and K. Ram Prabhakar and Ashwini Narayan and Sreevalli Sharma and Supreeth Guptha and Praveena Manjrekar and Murugan Chinnapattu and Vasanthi Ramachandran and Shahul P Hameed and Sudha Ravishankar and Monalisa Chatterji},
  journal={Antimicrobial agents and chemotherapy},
  year={2014},
  volume={58 10},
  pages={6165-71}
}
The Mur ligases play an essential role in the biosynthesis of bacterial peptidoglycan and hence are attractive antibacterial targets. A screen of the AstraZeneca compound library led to the identification of compound A, a pyrazolopyrimidine, as a potent inhibitor of Escherichia coli and Pseudomonas aeruginosa MurC. However, cellular activity against E. coli or P. aeruginosa was not observed. Compound A was active against efflux pump mutants of both strains. Experiments using an E. coli tolC… CONTINUE READING