UCE6, a new antitumor antibiotic with topoisomerase I-mediated DNA cleavage activity produced by actinomycetes: producing organism, fermentation, isolation and biological activity.

  title={UCE6, a new antitumor antibiotic with topoisomerase I-mediated DNA cleavage activity produced by actinomycetes: producing organism, fermentation, isolation and biological activity.},
  author={N. Fujii and F. Tanaka and Y. Yamashita and T. Ashizawa and S. Chiba and H. Nakano},
  journal={The Journal of antibiotics},
  volume={50 6},
A novel antitumor antibiotic, UCE6 (1,3,8,10,11-pentahydroxy-2-methyl-10-(2-oxo-4-hydroxypentyl)na phthacene-5, 12-dione) with topoisomerase I-mediated DNA cleavage activity, was isolated from the culture broth of actinomycetes strain UOE6. Addition of silicone oil antifoam agent, KS69 (2%), to the fermentation enhanced the production of UCE6 by approximately 3 fold. A total of 1.15 g of UCE6 was recovered as reddish orange crystals from a 100 liter fermentation supplemented with 2% KS69. UCE6… Expand
Production of antibacterial metabolites by strain no . 10 / 2 ( S . albovinaceus ) and media optimization studies for the maximum metabolite production
A new actinomycete strain no.10/2 (S.albovinaceus) with broad antibacterial and antifungal activities was isolated from soil sample of Palestine and characterized at our lab. Nutritional and culturalExpand
Production of Antibacterial and AntifungalMetabolites by (S.albovinaceus) Strain no.10/2 and MediaOptimization
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Non-camptothecin topoisomerase I active compounds as potential anticancer agents
The primary focus of this review is on those agents that most closely mimic the actions of camptothecin, the prototype topo I acting agent. Expand
A study of antifungal antibiotic production by Thermomonospora sp MTCC 3340 using full factorial design
The three independent variables, viz concentration of carbon source (glucose), concentration of nitrogen source (soybean meal) and temperature of incubation were found to be the most important forExpand
Total synthesis and development of bioactive natural products
  • K. Tatsuta
  • Chemistry, Medicine
  • Proceedings of the Japan Academy. Series B, Physical and biological sciences
  • 2008
The first total synthesis and development of a variety of bioactive natural products have been accomplished by using carbohydrates as a chiral source by using the “Big Four” antibiotics and a side-chain of cephem antibiotics. Expand
DNA Topoisomerase II Inhibitors
DNA topoisomerases represent a major focus of research not only for cancer chemotherapy, but also for gene regulation, cell cycle, mitosis, and chromosome structure. A number of reviews have beenExpand
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Characterization of a mammalian mutant with a camptothecin-resistant DNA topoisomerase I.
  • T. Andoh, K. Ishii, +4 authors K. Okada
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1987
It is established that DNA topoisomerase I is the cellular target of camptothecin and that DNATopoisomerases I is essential for the survival of mammalian cells. Expand
Synthesis of water-soluble (aminoalkyl)camptothecin analogs: inhibition of topoisomerase I and antitumor activity
Water-soluble analogues of the antitumor alkaloid camptothecin (1) were prepared in which aminoalkyl groups were introduced into ring A or B. Most of the analogues were prepared by oxidation ofExpand
Induction of mammalian DNA topoisomerase I and II mediated DNA cleavage by saintopin, a new antitumor agent from fungus.
Saintopin represents a new class of antitumor agent that can induce both mammalian DNA topoisomerase I and mammalianDNA topisomerase II mediated DNA cleavage. Expand
Correlation between the formation of cleavable complex with topoisomerase I and growth-inhibitory activity for saintopin-type antibiotics.
Immunoblot analysis using antibody against human topoisomerase I or II revealed that the protein linked to DNA in saintopin-type antibiotic-treated cells is most likely topoisomersase I, suggesting that saintopIn-type antibiotics interfere with topoisomes I in cells by trapping reversible topoisomease I/DNA cleavable complexes. Expand
Structure-activity study of the actions of camptothecin derivatives on mammalian topoisomerase I: evidence for a specific receptor site and a relation to antitumor activity.
Stereochemistry and the positions of substituents were found to be crucial for the presence or absence of effects on topoisomerase I, indicating that the compounds interact with an asymmetrical receptor site on the enzyme or enzyme-DNA complex. Expand
Metabolism and pharmacokinetics of the camptothecin analogue CPT-11 in the mouse.
In vitro incubation of CPT-11 in mouse serum or tissue homogenate enhanced the growth-inhibitory activity much more than that expected from the concentration of C PT-11, and it is suggested that the maintenance of plasma SN-38 concentration might be necessary for it to exhibit antitumor activity in vivo. Expand
Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I.
It is proposed that camptothecin blocks the rejoining step of the breakage-reunion reaction of mammalian DNA topoisomerase I, which results in the accumulation of a cleavable complex which resembles the transient intermediate proposed for eukaryotic DNATopoisomersase I. Expand
Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms
The primary structure of the peptidoglycan of Micromonospora is discussed and 45 strains of 12 genera examined, Actinoplanes, Ampullariella, Amorphosporangium, and Dactylospora species had a significant amount of glycolate in the whole cells. Expand
Chemical composition as a criterion in the classification of aerobic actinomycetes
The results show that whole-cell sugar patterns can usually be used to predict cell wall composition and that the combination of both criteria permit separation of aerobic actinomycetes into 10 taxonomically useful groups. Expand
Methods for characterization of Streptomyces species
The methods used by collaborators in the ISP for emendation of descriptions of type and neotype strains of the genus Streptomyces (Actinomycetales) are presented. Expand