U.V.C.-Induction of p53 activation and accumulation is dependent on cell cycle and pathways involving protein synthesis and phosphorylation

@article{Pitknen1998UVCInductionOP,
  title={U.V.C.-Induction of p53 activation and accumulation is dependent on cell cycle and pathways involving protein synthesis and phosphorylation},
  author={Kimmo Pitk{\"a}nen and Tarja Haapaj{\"a}rvi and Marikki Laiho},
  journal={Oncogene},
  year={1998},
  volume={16},
  pages={459-469}
}
Transcriptional activation and stabilization of p53 is a major response of mammalian cells to U.V.-light induced genetic damages, and possibly responsible for cell damage control. We have studied here by gel mobility shift and immunoblotting assays the activation and accumulation of p53 by U.V.C. and its dependency on cell cycle, protein synthesis and protein phosphorylation. In G0/G1 synchronized cells U.V.C.-induced p53 DNA-binding activity, but not its accumulation, whereas both events took… CONTINUE READING