Tyrosine phosphorylation of alpha tubulin in human T lymphocytes.

  title={Tyrosine phosphorylation of alpha tubulin in human T lymphocytes.},
  author={Steven C. Ley and W Verbi and Darryl J. Pappin and Brian Druker and Adelina A. Davies and Michael J. Crumpton},
  journal={European journal of immunology},
  volume={24 1},
N-terminal sequencing of the 55- and 50-kDa polypeptides affinity purified on a phosphotyrosine monoclonal antibody column from activated Jurkat T cells identified alpha and beta tubulin. Two-dimensional gel analysis indicated that alpha tubulin was directly phosphorylated on tyrosine. beta Tubulin was not detectably tyrosine phosphorylated but was precipitated by anti-phosphotyrosine (PTyr) antibody by virtue of its association with the alpha subunit as a heterodimer. Phosphotyrosyl alpha… CONTINUE READING