Tyrosine phosphorylation of HSC70 and its interaction with RFC mediates methotrexate resistance in murine L1210 leukemia cells.

@article{Liu2015TyrosinePO,
  title={Tyrosine phosphorylation of HSC70 and its interaction with RFC mediates methotrexate resistance in murine L1210 leukemia cells.},
  author={Tuoen Liu and Ratan Singh and Zechary Rios and Alok Bhushan and Mengxiong Li and Peter P. Sheridan and Shawn E. Bearden and James C. K. Lai and Senyo Agbenowu and Shousong Cao and Christopher K. Daniels},
  journal={Cancer letters},
  year={2015},
  volume={357 1},
  pages={
          231-241
        }
}
We previously identified and characterized a 66-68 kDa membrane-associated, tyrosine phosphorylated protein in murine leukemia L1210 cells as HSC70 which is a methotrexate (MTX)-binding protein. In order to further characterize the functional role of HSC70 in regulating MTX resistance in L1210 cells, we first showed that HSC70 colocalizes and interacts with reduced folate carrier (RFC) in L1210 cells by confocal laser scanning microscopy and Duolink in situ proximity ligation assay. The… Expand
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