Tyrosine-phosphorylation of AAV2 vectors and its consequences on viral intracellular trafficking and transgene expression.

@article{Zhong2008TyrosinephosphorylationOA,
  title={Tyrosine-phosphorylation of AAV2 vectors and its consequences on viral intracellular trafficking and transgene expression.},
  author={Li Li Zhong and Baozheng Li and Giridhararao Jayandharan and Cathryn S. Mah and Lakshmanan Govindasamy and Mavis Agbandje-McKenna and Roland W. Herzog and Kirsten A. K. Weigel-Van Aken and Jacqueline A. Hobbs and Sergei Zolotukhin and Nicholas Muzyczka and Arun Srivastava},
  journal={Virology},
  year={2008},
  volume={381 2},
  pages={194-202}
}
We have documented that epidermal growth factor receptor protein tyrosine kinase (EGFR-PTK) signaling negatively affects intracellular trafficking and transduction efficiency of recombinant adeno-associated virus 2 (AAV2) vectors. Specifically, inhibition of EGFR-PTK signaling leads to decreased ubiquitination of AAV2 capsid proteins, which in turn, facilitates viral nuclear transport by limiting proteasome-mediated degradation of AAV2 vectors. In the present studies, we observed that AAV… CONTINUE READING

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