Tyrosine phosphorylation and tyrosine kinase activity of the trk proto-oncogene product induced by NGF

@article{Kaplan1991TyrosinePA,
  title={Tyrosine phosphorylation and tyrosine kinase activity of the trk proto-oncogene product induced by NGF},
  author={David R Kaplan and Dionisio Mart{\'i}n-Zanca and Luis F. Parada},
  journal={Nature},
  year={1991},
  volume={350},
  pages={158-160}
}
NERVE growth factor (NGF) is a neurotrophic factor responsible for the differentiation and survival of sympathetic and sensory neurons as well as selective populations of cholinergic neurons1,2. NGF binds to specific cell-surface receptors but the mechanism for transduction of the neurotrophic signal is unknown. Several experiments using the NGF-responsive pheochromocytoma cell line, PC12, have implicated tyrosine phosphorylation in NGF-mediated responses, although no NGF-specific tyrosine… 
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Specific inhibition of NGF receptor tyrosine kinase activity by K-252a.
The trk tyrosine protein kinase mediates the mitogenic properties of nerve growth factor and neurotrophin-3
Neurotrophin signal transduction by the Trk receptor.
TLDR
The attempts to understand the mechanisms used by Trk to generate the many phenotypic responses of PC12 cells to NGF are summarized.
Tyrosine kinase activity coupled to the high-affinity nerve growth factor-receptor complex.
  • S. Meakin, E. Shooter
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1991
TLDR
It is suggested that the high-affinity NGF-receptor complex is a substrate of tyrosine kinase activity, which can be blocked by an agent that inhibits NGF (and not epidermal growth factor)-induced tyosine phosphorylation in PC12 cells, as well as N GF-induced neuronal differentiation of PC 12 cells.
Studying signal transduction in neuronal cells: the Trk/NGF system.
  • D. Kaplan
  • Biology
    Progress in brain research
  • 1998
The low-affinity p75 nerve growth factor (NGF) receptor mediates NGF-induced tyrosine phosphorylation.
TLDR
It is demonstrated that transfection of p75NGFR with mutations in the cytoplasmic domain resulted in an inability of NGF to elicit tyrosine phosphorylation of intracellular substrates, indicating that p75 NGFR is involved in initiating phosphorylated events by NGF.
TrkA Tyrosine Residues Involved in NGF‐induced Neurite Outgrowth of PC12 Cells
TLDR
Intacellular tyrosine residues of TrkA involved in nerve growth factor‐induced neurite outgrowth of PC12 cells are identified using site‐directed mutagenesis and a PC12 cell line expressing very low levels of endogenous Trka (PC12nnr5 cells).
Overexpression of the trk tyrosine kinase rapidly accelerates nerve growth factor-induced differentiation
Specificity of nerve growth factor signaling: Differential patterns of early tyrosine phosphorylation events induced by NGF, EGF, and bFGF
TLDR
Data presented suggest that the temporal pattern of induced phosphorylation, the nature of induced associations with other phosphoproteins, and cell type‐specific components may all contribute to the generation of NGF signaling specificity.
Activation of phosphatidylinositol-3 kinase by nerve growth factor involves indirect coupling of the trk proto-oncogene with src homology 2 domains
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References

SHOWING 1-10 OF 18 REFERENCES
Nerve growth factor induces protein-tyrosine phosphorylation.
  • P. Maher
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1988
TLDR
It is suggested that NGF and EGF induce protein-tyrosine phosphorylation in PC12 cells by different mechanisms.
Role of protein tyrosine phosphorylation in the NGF response
  • P. Maher
  • Biology, Chemistry
    Journal of neuroscience research
  • 1989
TLDR
It is suggested that protein tyrosine phosphorylation is one of the first events in the NGF pathway in PC12 cells but alone is not sufficient to induce morphological differentiation.
Direct activation of the serine/threonine kinase activity of raf-1 through tyrosine phosphorylation by the PDGF β-receptor
trkB, a novel tyrosine protein kinase receptor expressed during mouse neural development.
TLDR
In situ hybridization analysis of 14 and 18 day old mouse embryos indicates thattrkB transcripts are localized in the central (CNS) and peripheral (PNS) nervous systems, including brain, spinal cord, spinal and cranial ganglia, paravertebral trunk of the sympathetic nervous system and various innervation pathways, suggesting that trkB may code for a novel cell surface receptor involved in neurogenesis.
PDGF β-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex
Expression of the trk proto-oncogene is restricted to the sensory cranial and spinal ganglia of neural crest origin in mouse development.
TLDR
The data show that trk, a gene associated with malignancy in humans, is a specific marker for a set of neural crest-derived sensory neurons, and are consistent with the hypothesis that this proto-oncogene may have an important role in the development or phenotype of the neurons where it is expressed.
Molecular and biochemical characterization of the human trk proto-oncogene
TLDR
Results indicate that the product of the human trk locus is a novel tyrosine kinase cell surface receptor for an as yet unknown ligand.
Effect of protein synthesis inhibitors on growth factor activation of c-fos, c-myc, and actin gene transcription
TLDR
The results suggest that in PC12 cells c-fos transcription is activated by a protein-synthesis-independent mechanism, whereas c-myc stimulation requires new protein synthesis.
Synthesis, storage and release of acetylcholine by a noradrenergic pheochromocytoma cell line
TLDR
It is shown here that noradrenergic cultures of PC12 cells can also synthesise, store and release acetylcholine.
Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
  • L. Greene, A. Tischler
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1976
TLDR
A single cell clonal line which responds reversibly to nerve growth factor (NGF) has been established from a transplantable rat adrenal pheochromocytoma and should be a useful model system for neurobiological and neurochemical studies.
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