Tyrosinase immobilized magnetic nanobeads for the amperometric assay of enzyme inhibitors: application to the skin whitening agents.

@article{Sima2011TyrosinaseIM,
  title={Tyrosinase immobilized magnetic nanobeads for the amperometric assay of enzyme inhibitors: application to the skin whitening agents.},
  author={Veronica Harceaga Sima and St{\'e}phanie Patris and Zeynep Aydoğmuş and Ahmad Sarakbi and Robert Sandulescu and J H Kauffmann},
  journal={Talanta},
  year={2011},
  volume={83 3},
  pages={980-7}
}
The immobilization of tyrosinase onto glutaraldehyde activated streptavidine magnetic particles and subsequent retention onto a magnetized carbon paste electrode for the amperometric assay of tyrosinase inhibitors is described. Tyrosine was used as substrate as it is the first substrate in the melanogenesis process. The sensing mode is based on monitoring the decrease of the amperometric signal corresponding to the electrochemical reduction of dopaquinone enzymatically generated. This current… CONTINUE READING