Tyramide signal amplification (TSA) systems for the enhancement of ISH signals in cytogenetics.

@article{Bobrow2001TyramideSA,
  title={Tyramide signal amplification (TSA) systems for the enhancement of ISH signals in cytogenetics.},
  author={M N Bobrow and Phillip T. Moen},
  journal={Current protocols in cytometry},
  year={2001},
  volume={Chapter 8},
  pages={Unit 8.9}
}
This unit provides a series of techniques that dramatically improve the sensitivity of direct and indirect in situ detection. TSA is a peroxidase-based signal amplification system which is compatible with all in situ hybridization as well as immunocytochemical detection systems. The assay is performed on a glass slide and combines the use of fluorescent probes in either direct or indirect format using either an enzyme-labeled streptavidin or antibody. Protocols are provided for brightfield and… CONTINUE READING

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