Typing of human papillomaviruses by polymerase chain reaction amplification with L1 consensus primers and RFLP analysis.

@article{Lungu1992TypingOH,
  title={Typing of human papillomaviruses by polymerase chain reaction amplification with L1 consensus primers and RFLP analysis.},
  author={Octavian Lungu and Thomas Wright and Saul Silverstein},
  journal={Molecular and cellular probes},
  year={1992},
  volume={6 2},
  pages={145-52}
}
Human papillomaviruses (HPV) cause benign and malignant lesions of the epithelial and mucosal surfaces. Certain virus types are associated with cervical carcinomas, while others are associated with benign condylomata. We have developed a rapid method for determining HPV type that is based on restriction fragment length polymorphism (RFLP) analysis within the L1 region of HPVs that is amplified by PCR using the consensus primers described by Manos et al. Analysis of the product generated by PCR… CONTINUE READING

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