Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis.

@article{Bedendo2000TypingOE,
  title={Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis.},
  author={J Bedendo and Ant{\^o}nio Carlos Campos Pignatari},
  journal={Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas},
  year={2000},
  volume={33 11},
  pages={1269-74}
}
Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eight Enterococcus faecium isolates from Stanford University and 12 from São Paulo Hospital were studied… CONTINUE READING

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