Type VI collagen in the rat testis: monoclonal antibody, isolation, and localization during development.

@article{Sawada1994TypeVC,
  title={Type VI collagen in the rat testis: monoclonal antibody, isolation, and localization during development.},
  author={Hajime Sawada and Futoshi Yazama},
  journal={Biology of reproduction},
  year={1994},
  volume={50 3},
  pages={
          702-10
        }
}
To investigate the components of the seminiferous tubule extracellular matrix (ST-ECM), monoclonal antibodies were raised against ST-ECM. One of these (15B6) recognized the nonreduced form of type VI collagen. With this antibody as a probe, type VI collagen was isolated from an SDS-urea extract of ST-ECM. It took a tetrameric form, and upon reduction dissociated into a major 140-kDa band and two bands at 190 and 210 kDa. Developmental changes in the distribution of type VI collagen were… CONTINUE READING

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