Type IIs restriction based combinatory modulation technique for metabolic pathway optimization

Abstract

BACKGROUND One of the most important research subjects of metabolic engineering is pursuing a balanced metabolic pathway, which is the basis of an efficient cell factory. In this work, we dedicated to develop a simple and efficient technique to modulate expression of multiple genes simultaneously, and select for the optimal regulation pattern. RESULTS A… (More)
DOI: 10.1186/s12934-017-0659-z

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