Type III restriction enzymes need two inversely oriented recognition sites for DNA cleavage

@article{Meisel1992TypeIR,
  title={Type III restriction enzymes need two inversely oriented recognition sites for DNA cleavage},
  author={A. Meisel and T. Bickle and Detlev H. Kriiger and C. Schroeder},
  journal={Nature},
  year={1992},
  volume={355},
  pages={467-469}
}
TYPE III restriction/modification enzymes recognize short, non-palindromic sequences that can be methylated on only one strand, with the paradoxical consequence that during replication of what is in effect hemimethylated DNA totally unmodified sites arise1. Why the unmodified sites are not subject to suicidal restriction was not clear. Here we show that restriction requires two unmodified recognition sites that can be separated by different distances but which must be in inverse orientation… Expand

Topics from this paper

Type III restriction enzymes communicate in 1D without looping between their target sites
Type III restriction-modification enzymes: a historical perspective
Mechanistic insights into type III restriction enzymes.
DNA communications by Type III restriction endonucleases--confirmation of 1D translocation over 3D looping.
Unidirectional translocation from recognition site and a necessary interaction with DNA end for cleavage by Type III restriction enzyme.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 10 REFERENCES
Purification and properties of the P15 specific restriction endonuclease from Escherichia coli.
Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I.
  • S. Tabor, C. Richardson
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1989
M.EcoP15 methylates the second adenine in its recognition sequence.