Type III restriction endonucleases are heterotrimeric: comprising one helicase–nuclease subunit and a dimeric methyltransferase that binds only one specific DNA

@inproceedings{Butterer2014TypeIR,
  title={Type III restriction endonucleases are heterotrimeric: comprising one helicase–nuclease subunit and a dimeric methyltransferase that binds only one specific DNA},
  author={Annika Butterer and Christian Pernstich and Rachel M. Smith and Frank Sobott and Mark D. Szczelkun and J{\'u}lia T{\'o}th},
  booktitle={Nucleic acids research},
  year={2014}
}
Fundamental aspects of the biochemistry of Type III restriction endonucleases remain unresolved despite being characterized by numerous research groups in the past decades. One such feature is the subunit stoichiometry of these hetero-oligomeric enzyme complexes, which has important implications for the reaction mechanism. In this study, we present a series of results obtained by native mass spectrometry and size exclusion chromatography with multi-angle light scattering consistent with a 1:2… CONTINUE READING
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