Type I callus as a bombardment target for generating fertile transgenic maize (Zea mays L.)


To develop a less genotype-dependent maize-transformation procedure, we used 10-month-old Type I callus as target tissue for microprojectile bombardment. Twelve transgenic callus lines were obtained from two of the three anther-culture-derived callus cultures representing different gentic backgrounds. Multiple fertile transgenic plants (T0) were regenerated… (More)
DOI: 10.1007/BF00193211


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