Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells

  title={Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells},
  author={Isao Miyoshi and Ichiro Kubonishi and Shizuo Yoshimoto and Tadaatsu Akagi and Yuji Ohtsuki and Yukimasa Shiraishi and Kin-ya Nagata and Yorio Hinuma},
A recent nationwide survey of the lymphocyte subpopulations of leukaemia and lymphoma in Japan has disclosed a high incidence of adult T-cell leukaemia (ATL)1. One of the striking features of this disease is the clustering of patients in the southwestern part of Japan1,2. We have established a continuous culture line of leukaemic T cells from a patient with ATL3,4. This cell line, MT-1, was found to carry type C virus particles and ATL-associated antigens (ATLA) which specifically reacted with… 

Immunoagglutination of type-C virus particles in a human T-cell line by serum supplementation from patients with adult T-cell leukemia

Results provide direct evidence for the specific reactivity of ATL patients' sera with type-C virus particles in the MT-2 cell line at the ultrastructural level.

Molecular Features of the Human T-Cell Leukemia Virus

It has been established by seroepi-demiology and molecular studies that HTLV-I is the etiologic agent of ATL.

Transmission of human T-cell leukemia virus (HTLV) into human cord blood T cells.

The transmission of HTLV into human cord blood T cells is described and the HTLV-induced changes in cell growth and surface phenotype are shown and evidence for changes in expression of certain genes is shown.

Homology of human T-cell leukaemia virus envelope gene with class I HLA gene

Homology between the envelope gene region of HTLV and the region of an HLA-B locus gene which codes for the extracellular portion of a class I histocompatibility antigen is shown.

Characterization of two human lymphoid cell lines producing human T-lymphotropic virus type I (HTLV-I) isolated from patients with HTLV-I-associated myelopathy or encephalopathy

The facts indicate that HTLV-I-infected cells were present in CSF of these two patients, and suggest that neurological disorders associated with HTLV -I may not be restricted to myelopathy and may include brain abnormalities.

Characterization of a novel HTLV-infected cell line.

B lymphocytes can be infected with HTLV, although no disease of HTLV-infected B lymphocytes has been reported and isolated a B lymphocyte clone from ME that expresses cytoplasmic immunoglobulin and p19 and p24 antigens, contains the EBV and HTLV genomes, and can transform T lymphocytes from normal volunteers.

Transformed T lymphocytes infected by a novel isolate of human T cell leukemia virus type II.

Electron microscopic examination of the CM cell cultures and the transformed CBL do not require exogenous interleukin 2, have T cell cytochemical features and mature T helper phenotypes, and exhibit minimal T helper and profound T suppressor activity on pokeweed mitogen-stimulated differentiation of normal B cells, which may represent properties of all HTLV-II-infected T cells.

Isolation of human T-cell leukemia virus in acquired immune deficiency syndrome (AIDS).

Whether or not HTLV-I or other retroviruses of this family with T-cell tropism cause AIDS, it is possible that patients from whom the virus can be isolated can also transmit it to others.

Cell lines producing human T-cell lymphoma virus show altered HLA expression

In all HTLV positive cells, with demonstrable provirus replication, altered HLA alloantigen expression was observed and may be explained by the observations reported in the accompanying paper8 which shows homology between the envelope gene region of HTLV and the region of an HLA-B locus gene which codes for the extracellular portion of a class I histocompatibility antigen.



Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma

The number of these particle-associated proteins is consistent with the expected proteins of a retrovirus, but the sizes of some are distinct from those of most known retroviruses of the primate subgroups.

Adult T-cell leukemia: clinical and hematologic features of 16 cases.

Clinical and hematologic studies of 16 adult patients whose leukemic cells had Tcell markers from Japan, where the incidence of various lymphoproliferative diseases differs considerably from that in Western countries, discuss the relation between cases and other subacute or chronic adult T-ceIl malignancies.

Humans have antibodies capable of recognizing oncoviral glycoproteins: demonstration that these antibodies are formed in response to cellular modification of glycoproteins rather than as consequence of exposure to virus.

It is shown that antibodies capable of precipitating a wide variety of oncoviral glycoproteins can be demonstrated under certain assay conditions, and the findings not only demonstrate that the antibodies were directed against cellular rather than the virus-coded antigenic determinants but also exclude the possibility that this immune response was elicited as a consequence ofoncovirus exposure.