Type 3 lodothyronine deiodinase: cloning, in vitro expression, and functional analysis of the placental selenoenzyme.

@article{Salvatore1995Type3L,
  title={Type 3 lodothyronine deiodinase: cloning, in vitro expression, and functional analysis of the placental selenoenzyme.},
  author={Domenico Salvatore and Susan C. Low and Meera Berry and Alberto Luis Maia and John W. Harney and Walburga Croteau and Donald L. St. Germain and P. Reed Larsen},
  journal={The Journal of clinical investigation},
  year={1995},
  volume={96 5},
  pages={
          2421-30
        }
}
Type 3 iodothyronine deiodinase (D3) catalyzes the conversion of T4 and T3 to inactive metabolites. It is highly expressed in placenta and thus can regulate circulating fetal thyroid hormone concentrations throughout gestation. We have cloned and expressed a 2.1-kb human placental D3 cDNA which encodes a 32-kD protein with a Km of 1.2 nM for 5 deiodination of T3 and 340 nM for 5' deiodination of reverse T3. The reaction requires DTT and is not inhibited by 6n-propylthiouracil. We quantitated… CONTINUE READING

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