Ty element transposition: Reverse transcriptase and virus-like particles

@article{Garfinkel1985TyET,
  title={Ty element transposition: Reverse transcriptase and virus-like particles},
  author={David J. Garfinkel and Jef D. Boeke and Gerald R. Fink},
  journal={Cell},
  year={1985},
  volume={42},
  pages={507-517}
}
Reverse transcriptase activity and Ty RNA are associated with virus-like particles in yeast
TLDR
The reverse transcriptase activity that, until now, has been presumed to mediate Ty transposition and show that it is sequestered in virus-like particles that also contain Ty RNA is identified.
Characterization of products of TY1-mediated reverse transcription in Saccharomyces cerevisiae
TLDR
Analysis of the extrachromosomal Tyspecific nucleic acid molecules associated with overproduced Ty virus-like particles identified several reverse transcribed cDNA strands that resemble the characteristic intermediates of the reverse transcription process described for authentic retroviruses.
The expression of hybrid HIV:Ty virus-like particles in yeast
TLDR
It is demonstrated that pl fusion proteins, comprising most of pi and part of human immunodeficiency virus (HIV) protein gp120, form hybrid HIV:Ty-VLPs, which provide a rapid means of preparing and evaluating HIV antigens for a variety of immunological purposes.
Processing of TY1 proteins and formation of Ty1 virus-like particles in Saccharomyces cerevisiae
TLDR
Functional properties of putative proteins encoded by the yeast transposable element, Ty1, by overexpression of TY genes support the hypothesis that TYB is expressed as a TYA/TYB fusion protein which is processed by a TYB-encoded protease activity.
Functional organization of the retrotransposon Ty from Saccharomyces cerevisiae: Ty protease is required for transposition.
TLDR
Results indicate that the Ty protease, like its retroviral counterpart, plays an important role in particle assembly, replication, and transposition of these elements.
Functional organization of the retrotransposon Ty from Saccharomyces cerevisiae: Ty protease is required for transposition
We used several mutations generated in vitro to further characterize the functions of the products encoded by the TyB gene of the transpositionally active retrotransposon TyH3 from Saccharomyces
Efficient homologous recombination of Ty1 element cDNA when integration is blocked.
TLDR
Ty1 cDNA is utilized much more efficiently for homologous recombination when IN-mediated integration is blocked, and multimeric insertions occurred without altering the levels of total Ty1 RNA, virus-like particle-associated RNA or cDNA, Ty1 capsid proteins, or IN.
Efficient homologous recombination of Ty1 element cDNA when integration is blocked
Integration of the yeast retrotransposon Ty1 into the genome requires the self-encoded integrase (IN) protein and specific terminal nucleotides present on full-length Ty1 cDNA. Ty1 mutants with
Ty3 GAG3 and POL3 genes encode the components of intracellular particles
TLDR
Investigation of the nucleocapsid particle of a transpositionally active gypsylike retrotransposon showed the presence of Ty3 nucleoprotein complexes, or viruslike particles, that migrated on linear sucrose gradients with a size of 156S.
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