The purpose of this study was to evaluate the long-term retention and effect on caries rates of pit and fissure sealants containing fluoride (UltraSeal XT and FluoroShield). These sealants were placed in vivo on opposite sides of the arch in adolescent patients in a private practice. A total of 470 sealants were placed on permanent molars and premolars. After two years, 74.3 percent of the sample was available for recall. The total retention rate (Category A) was 96.3 percent for UltraSeal XT and 91.4 percent for FluoroShield. There were no new pit and fissure carious lesions over the two years of the study. The results for the UltraSeal XT is the highest retention rate yet documented.