Two-way communication between SecY and SecA suggests a Brownian ratchet mechanism for protein translocation

@inproceedings{Allen2016TwowayCB,
  title={Two-way communication between SecY and SecA suggests a Brownian ratchet mechanism for protein translocation},
  author={William John Allen and Robin Adam Corey and Peter Oatley and Richard B. Sessions and Steve A Baldwin and Sheena E Radford and Roman Tuma and Ian Collinson},
  booktitle={eLife},
  year={2016}
}
The essential process of protein secretion is achieved by the ubiquitous Sec machinery. In prokaryotes, the drive for translocation comes from ATP hydrolysis by the cytosolic motor-protein SecA, in concert with the proton motive force (PMF). However, the mechanism through which ATP hydrolysis by SecA is coupled to directional movement through SecYEG is unclear. Here, we combine all-atom molecular dynamics (MD) simulations with single molecule FRET and biochemical assays. We show that ATP… CONTINUE READING