Two types of tumor necrosis factor-alpha in bluefin tuna (Thunnus orientalis) genes: Molecular cloning and expression profile in response to several immunological stimulants.

@article{Kadowaki2009TwoTO,
  title={Two types of tumor necrosis factor-alpha in bluefin tuna (Thunnus orientalis) genes: Molecular cloning and expression profile in response to several immunological stimulants.},
  author={Takeshi Kadowaki and Hideaki Harada and Yoshifumi Sawada and Chie Kohchi and Gen-ichiro Soma and Yukinori Takahashi and Hiroyuki Inagawa},
  journal={Fish & shellfish immunology},
  year={2009},
  volume={27 5},
  pages={585-94}
}
Tumor necrosis factor-alpha (TNF-alpha) is a key inflammatory mediator and has also the potential as a prominent biomarker of innate immunity. In this study, we identified and characterized TNF-alpha from bluefin tuna, which is an important cultured species. Two types of TNF-alpha were also cloned incidentally (TNF1 and TNF2). The open reading frame of TNF1 and TNF2 cDNA encoded 247 and 245 amino acids, respectively. The amino acid sequence identity among sea perch, red sea bream, and tiger… CONTINUE READING

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