Two substrate sites in the renal Na+-d-glucose cotransporter studied by model analysis of phlorizin binding and-d-glucose transport measurements

@article{Koepsell1990TwoSS,
  title={Two substrate sites in the renal Na+-d-glucose cotransporter studied by model analysis of phlorizin binding and-d-glucose transport measurements},
  author={Hermann Koepsell and G{\"u}nter Fritzsch and Klaus Korn and Andrzej Madrala},
  journal={The Journal of Membrane Biology},
  year={1990},
  volume={114},
  pages={113-132}
}
Time courses of phlorizin binding to the outside of membrane vesicles from porcine renal outer cortex and outer medulla were measured and the obtained families of binding curves were fitted to different binding models. To fit the experimental data a model with two binding sites was required. Optimal fits were obtained if a ratio of low and high affinity phlorizin binding sites of 1:1 was assumed. Na+ increased the affinity of both binding sites. By an inside-negative membrane potential the… CONTINUE READING

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