Two-step purification of mouse kidney ornithine decarboxylase.

  title={Two-step purification of mouse kidney ornithine decarboxylase.},
  author={Midori Nishiyama and Senya Matsufuji and Rina Kanamoto and Masako Takano and Yasuko Murakami and Shin-ichi Hayashi},
  journal={Preparative biochemistry},
  volume={18 2},
We developed a simple two-step purification procedure for ornithine decarboxylase (ODC, EC, consisting of DEAE-Cellulofine chromatography and affinity chromatography on a HO-101 monoclonal anti-rat liver ODC antibody-Affi-Gel 10 column. By this method, ODC was purified 1700-fold to homogeneity with about 80% yield from the kidney of ICR mice treated with testosterone enanthate. The final specific activity range between 1.0 x 10(6)-1.4 x 10(6) nmol/ protein. On SDS-polyacrylamide… CONTINUE READING