Two-step chromatographic method for separation and purification of nerve growth factor from venom of Chinese cobra.

@article{Bian2004TwostepCM,
  title={Two-step chromatographic method for separation and purification of nerve growth factor from venom of Chinese cobra.},
  author={Liujiao Bian and Peng Wu and Xiaoyan Yang},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2004},
  volume={805 1},
  pages={119-25}
}
By selecting the different combination schemes, a simple, fast and highly efficient method for separation and purification of nerve growth factor (NGF) from venom of Chinese cobra is reported in this paper. This purification process consists of a two-step chromatographic separation on DEAE-Sepharose F.F. anion-exchange medium followed by a Sephadex G-50 gel filtration. On reducing and non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the nerve growth factor… CONTINUE READING

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