Two-stage Off-Gel isoelectric focusing: protein followed by peptide fractionation and application to proteome analysis of human plasma.

@article{Heller2005TwostageOI,
  title={Two-stage Off-Gel isoelectric focusing: protein followed by peptide fractionation and application to proteome analysis of human plasma.},
  author={Manfred Heller and Philippe E Michel and Patrick Morier and David Crettaz and Christian Wenz and J Tissot and Fr{\'e}d{\'e}ric Reymond and Joel S Rossier},
  journal={Electrophoresis},
  year={2005},
  volume={26 6},
  pages={1174-88}
}
This paper presents the recently introduced Off-Gel electrophoresis (OGE) technology as a versatile tool to reproducibly fractionate intact proteins and peptides into discrete liquid fractions. The coupling of two stages of OGE, i.e., the separation of intact proteins in a first-stage followed by fractionation of peptides derived from each protein fraction after proteolysis in a second stage, results in an array of 15 x 15 fractions that are directly amenable to additional peptide fractionation… CONTINUE READING

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