Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein.

@article{Uotila1981TwositeSE,
  title={Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein.},
  author={M H Uotila and Erkki Ruoslahti and Eva Engvall},
  journal={Journal of immunological methods},
  year={1981},
  volume={42 1},
  pages={11-5}
}
Monoclonal antibodies reacting with different antigenic determinants on the alpha-fetoprotein (AFP) molecule were used to develop a simplified 'sandwich' ELISA (enzyme-linked immunospecific assay) in which one monoclonal antibody was immobilized and another labeled with an enzyme. Due to their different specificities the antibody attached to the solid phase and the one conjugated to the enzyme did not compete for binding to AFP making it possible to complete the two antigen-antibody reactions… CONTINUE READING
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