Two-site immunochemiluminometric assay of intact human parathyrin in serum with use of a tracer peptide purified by reversed-phase high-performance liquid chromatography.

@article{Bhler1989TwositeIA,
  title={Two-site immunochemiluminometric assay of intact human parathyrin in serum with use of a tracer peptide purified by reversed-phase high-performance liquid chromatography.},
  author={Univ.-Prof. Dr. Kornelia B{\"o}hler and Eberhard Blind and Gunther Vogel and Walter Hitzler and Dagmar Flentje and Heinrich Schmidt-Gayk},
  journal={Clinical chemistry},
  year={1989},
  volume={35 2},
  pages={215-22}
}
For this two-site immunochemiluminometric assay of intact human parathyrin (hPTH), the luminescent tracer was synthetic hPTH(53-84), conjugated via succinimide linkage to (aminobutyl)ethyl-isoluminol hemisuccinimide (abei-h). Purification of the labeled hPTH(53-84) by reversed-phase high-performance liquid chromatography allowed isolation of the conjugate having the highest incorporation of abei-h, 1.6 mol per mole of hPTH(53-84). The solid-phase antibody directed against the N-terminal part of… CONTINUE READING