Two-round allele specific-polymerase chain reaction: a simple and highly sensitive method for JAK2V617F mutation detection.

@article{Kannim2009TworoundAS,
  title={Two-round allele specific-polymerase chain reaction: a simple and highly sensitive method for JAK2V617F mutation detection.},
  author={Supattra Kannim and Wanna Thongnoppakhun and Chirayu Udomsakdi Auewarakul},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={2009},
  volume={401 1-2},
  pages={
          148-51
        }
}
BACKGROUND Philadelphia chromosome (Ph(1))-negative myeloproliferative disorders (MPD) are hematopoietic stem cell disorders characterized by extensive proliferation of myeloid blood cells. JAK2V617F has recently been identified in the majority of Ph(1)-negative MPD and becomes an essential diagnostic marker. METHODS To screen for JAK2V617F, a two-round allele specific-polymerase chain reaction (AS-PCR) was developed and compared to PCR-restriction fragment length polymorphism (PCR-RFLP… CONTINUE READING
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