Two roles for the DNA recognition site of the Klebsiella aerogenes nitrogen assimilation control protein.

@article{Pomposiello1998TwoRF,
  title={Two roles for the DNA recognition site of the Klebsiella aerogenes nitrogen assimilation control protein.},
  author={Pablo J. Pomposiello and Brian K. Janes and Robert A. Bender},
  journal={Journal of bacteriology},
  year={1998},
  volume={180 3},
  pages={578-85}
}
The nitrogen assimilation control protein (NAC) binds to a site within the promoter region of the histidine utilization operon (hutUH) of Klebsiella aerogenes, and NAC bound at this site activates transcription of hutUH. This NAC-binding site was characterized by a combination of random and directed DNA mutagenesis. Mutations that abolished or diminished in vivo transcriptional activation by NAC were found to lie within a 15-bp region contained within the 26-bp region protected by NAC from… CONTINUE READING

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