Two-photon targeted recording of GFP-expressing neurons for light responses and live-cell imaging in the mouse retina

@article{Wei2010TwophotonTR,
  title={Two-photon targeted recording of GFP-expressing neurons for light responses and live-cell imaging in the mouse retina},
  author={Wei Wei and Justin Blake Elstrott and Marla B Feller},
  journal={Nature Protocols},
  year={2010},
  volume={5},
  pages={1347-1352}
}
Cell type–specific green fluorescent protein (GFP) expression in the retina has been achieved in an expanding repertoire of transgenic mouse lines, which are valuable tools for dissecting the retinal circuitry. However, measuring light responses from GFP-labeled cells is challenging because single-photon excitation of GFP easily bleaches photoreceptors. To circumvent this problem, we use two-photon excitation at 920 nm to target GFP-expressing cells, followed by electrophysiological recording… CONTINUE READING
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