Two-photon microscopy as a tool to study blood flow and neurovascular coupling in the rodent brain.

@article{Shih2012TwophotonMA,
  title={Two-photon microscopy as a tool to study blood flow and neurovascular coupling in the rodent brain.},
  author={Andy Y. Shih and Jonathan Daniel Driscoll and Patrick J. Drew and Nozomi Nishimura and Chris B. Schaffer and David Kleinfeld},
  journal={Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism},
  year={2012},
  volume={32 7},
  pages={
          1277-309
        }
}
The cerebral vascular system services the constant demand for energy during neuronal activity in the brain. Attempts to delineate the logic of neurovascular coupling have been greatly aided by the advent of two-photon laser scanning microscopy to image both blood flow and the activity of individual cells below the surface of the brain. Here we provide a technical guide to imaging cerebral blood flow in rodents. We describe in detail the surgical procedures required to generate cranial windows… CONTINUE READING
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