Two-photon laser scanning fluorescence microscopy.

@article{Denk1990TwophotonLS,
  title={Two-photon laser scanning fluorescence microscopy.},
  author={Winfried Denk and James H. Strickler and Watt W. Webb},
  journal={Science},
  year={1990},
  volume={248 4951},
  pages={
          73-6
        }
}
Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy. The excitation of fluorophores having single-photon absorption in the ultraviolet with a stream of strongly focused subpicosecond pulses of red laser light has made possible fluorescence images of living cells and other microscopic objects. The fluorescence emission increased quadratically with the excitation intensity so that fluorescence… 

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