Two-photon laser scanning fluorescence microscopy.

@article{Denk1990TwophotonLS,
  title={Two-photon laser scanning fluorescence microscopy.},
  author={Winfried Denk and James H. Strickler and Watt W. Webb},
  journal={Science},
  year={1990},
  volume={248 4951},
  pages={
          73-6
        }
}
Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy. The excitation of fluorophores having single-photon absorption in the ultraviolet with a stream of strongly focused subpicosecond pulses of red laser light has made possible fluorescence images of living cells and other microscopic objects. The fluorescence emission increased quadratically with the excitation intensity so that fluorescence… Expand
Development of a confocal laser scanning fluorescence microscope using two-photon excitation in combination with time-gated detection
Fluorescent molecules having single-photon absorption in the blue and the UV can be excited with infra-red light via a process known as two-photon excitation. The combination of this technique withExpand
Three-photon excitation in fluorescence microscopy.
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Three-photon excitation fluorescence axial images are shown of polystyrene beads stained with the fluorophore 2,5-bis(4-biphenyl)oxazole (BBO) using a mode-locked titanium sapphire laser, proving the feasibility of scanning fluorescence microscopy by three-ph photon excitation. Expand
Three-photon light-sheet fluorescence microscopy.
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The first demonstration of three-photon excitation light-sheet fluorescence microscopy is presented, using a conventional femtosecond pulsed laser at 1000 nm wavelength for the imaging of 450 μm diameter cellular spheroids. Expand
Two-Photon Microscopy in Highly Scattering Tissue
Molecular excitation by two-photon absorption and the subsequent fluorescence have proved to be a useful tool for imaging biological systems using laser-scanning microscopy (Denk et al., 1990). InExpand
Combined Raman and continuous-wave-excited two-photon fluorescence cell imaging.
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With this microscope fast image acquisition with fluorescence imaging can be used to select areas of interest for subsequent chemical analysis with spontaneous Raman imaging. Expand
Two-photon excitation fluorescence microscopy.
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Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging and is a novel method to trigger localized photochemical reactions. Expand
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Multi-photon microscopy uses a different principle in exciting fluorochromes from conventional epi-fluorescence microscopy and confocal laser scanning fluorescence microscopy. In multi-photonExpand
Two-photon excitation in scanning laser microscopy (Abstract Only)
Two-photon excitation in scanning laser fluorescence microscopy provides axial resolution and rejection of out of focus background similar to that provided by confocal microscopy. Two- photonExpand
Combined scanning optical coherence and two-photon-excited fluorescence microscopy.
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Simultaneous imaging of cell nuclei with OCM and TPE is demonstrated in live drosophila embryos and both modes provide rapid en-face imaging with submicrometer resolution. Expand
Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging
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The calculation of point spread functions shows that the visible-wavelength two-photon excitation provides the fundamental improvement of spatial resolution compared to conventional confocal microscopy. Expand
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Two - photon laser microscopy
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