Two-photon imaging of glutathione levels in intact brain indicates enhanced redox buffering in developing neurons and cells at the cerebrospinal fluid and blood-brain interface.

@article{Sun2006TwophotonIO,
  title={Two-photon imaging of glutathione levels in intact brain indicates enhanced redox buffering in developing neurons and cells at the cerebrospinal fluid and blood-brain interface.},
  author={Xiaojian Sun and Andy Y. Shih and Helge C. Johannssen and Heidi Erb and Ping Li and Timothy H. Murphy},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 25},
  pages={17420-31}
}
Glutathione is the major cellular thiol present in mammalian cells and is critical for maintenance of redox homeostasis. However, current assay systems for glutathione lack application to intact animal tissues. To map the levels of glutathione in intact brain with cellular resolution (acute tissue slices and live animals), we have used two-photon imaging of monochlorobimane fluorescence, a selective enzyme-mediated marker for reduced glutathione. Previously, in vitro experiments using purified… CONTINUE READING
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