Two-photon image correlation spectroscopy and image cross-correlation spectroscopy.

@article{Wiseman2000TwophotonIC,
  title={Two-photon image correlation spectroscopy and image cross-correlation spectroscopy.},
  author={Paul W Wiseman and Jeff A. Squier and Mark H. Ellisman and Kevin R Wilson},
  journal={Journal of microscopy},
  year={2000},
  volume={200 Pt 1},
  pages={
          14-25
        }
}
We introduce two-photon image correlation spectroscopy (ICS) using a video rate capable multiphoton microscope. We demonstrate how video rate two-photon microscopic imaging and image correlation analysis may be combined to measure molecular transport properties over ranges typical of biomolecules in membrane environments. Using two-photon ICS, we measured diffusion coefficients as large as 10(-8) cm2 s(-1) that matched theoretical predictions for samples of fluorescent microspheres suspended in… CONTINUE READING

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