Two-photon fluorescence bioimaging with an all-semiconductor laser picosecond pulse source.

@article{Kuramoto2007TwophotonFB,
  title={Two-photon fluorescence bioimaging with an all-semiconductor laser picosecond pulse source.},
  author={Masaru Kuramoto and Nobuyoshi Kitajima and Hengchang Guo and Yuji Furushima and Masao Ikeda and Hiroyuki Yokoyama},
  journal={Optics letters},
  year={2007},
  volume={32 18},
  pages={2726-8}
}
We have demonstrated successful two-photon excitation fluorescence bioimaging using a high-power pulsed all-semiconductor laser. Toward this purpose, we developed a pulsed light source consisting of a mode-locked laser diode and a two-stage diode laser amplifier. This pulsed light source provided optical pulses of 5 ps duration and having a maximum peak power of over 100 W at a wavelength of 800 nm and a repetition frequency of 500 MHz. 
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