Two-photon excited fluorescence enhancement with broadband versus tunable femtosecond laser pulse excitation.

@article{Wang2012TwophotonEF,
  title={Two-photon excited fluorescence enhancement with broadband versus tunable femtosecond laser pulse excitation.},
  author={Chao Wang and Alvin T. Yeh},
  journal={Journal of biomedical optics},
  year={2012},
  volume={17 2},
  pages={025003}
}
The inverse relationship between two-photon excited fluorescence (TPEF) and laser pulse duration suggests that two-photon microscopy (TPM) performance may be improved by decreasing pulse duration. However, for ultrashort pulses of sub-10 femtosecond (fs) in duration, its spectrum contains the effective gain bandwidth of Ti:Sapphire and its central wavelength is no longer tunable. An experimental study was performed to explore this apparent tradeoff between untuned sub-10 fs transform-limited… CONTINUE READING

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