Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo

@article{Mittmann2011TwophotonCI,
  title={Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo},
  author={Wolfgang Mittmann and Damian J. Wallace and Uwe Czubayko and Jan T Herb and Andreas T. Schaefer and Loren L. Looger and Winfried Denk and Jason N. D. Kerr},
  journal={Nature Neuroscience},
  year={2011},
  volume={14},
  pages={1089-1093}
}
Multiphoton imaging (MPI) is widely used for recording activity simultaneously from many neurons in superficial cortical layers in vivo. We combined regenerative amplification multiphoton microscopy (RAMM) with genetically encoded calcium indicators to extend MPI of neuronal population activity into layer 5 (L5) of adult mouse somatosensory cortex. We found… CONTINUE READING