Two olfactory marker proteins in Xenopus laevis

  title={Two olfactory marker proteins in Xenopus laevis},
  author={Patricia Roessler and Mario Mezler and Heinz Breer},
  journal={Journal of Comparative Neurology},
Mature olfactory receptor neurons of mammals are characterized by the expression of the highly conserved olfactory marker protein (OMP) encoded by single copy genes. In Xenopus laevis, two homologous genes encoding olfactory marker proteins have been identified that share a sequence identity with mammalian OMPs of about 50%. Sequence comparison revealed significant variability in the N‐terminus and C‐terminus regions; in contrast, two internal domains were highly conserved between amphibian and… 
Selective targeting of zebrafish olfactory receptor neurons by the endogenous OMP promoter
The olfactory nervous system of fish, in particular zebrafish, has become a valid model for that of higher vertebrates. However, no genetic markers for olfactory specific cell types, e.g. the
The functional relevance of olfactory marker protein in the vertebrate olfactory system: a never-ending story
A broader approach to diverse cellular systems might help unravel OMP’s functions and mechanisms of action inside and outside the nose.
Expression of olfactory receptors during development in Xenopus laevis.
Using RT-PCR and in situ hybridization, it was found that expression of Xenopus olfactory marker protein and of class I receptors, which are thought to be responsible for the perception of water-soluble odorants, was detectable as early as stage 32, less than 2 days after fertilization.
Ancestral amphibian v2rs are expressed in the main olfactory epithelium
It is reported here that, strikingly different from both lineages, the v2r gene family of the amphibian Xenopus laevis is expressed in the main olfactory as well as the vomeronasal epithelium, presenting an excellent opportunity to study the transition of v2R gene expression during evolution of higher vertebrates.
Cloning of an olfactory sensory neuron–specific protein in the land snail (Eobania vermiculata)
Sequence and expression pattern analogy of land snail protein with olfactory marker protein (OMP) from vertebrates suggest that the land snailprotein is an OMP–like protein.
Olfactory marker protein (OMP) regulates formation and refinement of the olfactory glomerular map
It is reported that OMP deletion leads to formation of glomeruli with axons from heterogeneous OSNs due to local axonal mistargeting, revealing an unexpected role for OMP in the formation and refinement of the glomerular map.
Differential antigen expression during metamorphosis in the tripartite olfactory system of the African clawed frog, Xenopus laevis
The E7 antibody can distinguish the three areas of the olfactory epithelium, allowing measurement of sensory epithelia volume, and serves as an excellent marker for the changes in the sensory epithellium that occur during metamorphosis.
Distinct functions of two olfactory marker protein genes derived from teleost-specific whole genome duplication
This study finds distinct expression patterns of zebrafish OMP1 and 2 at the tissue and cellular level and provides a good example for analyzing a functional subdivision of the teleost OE and eyes as revealed by 3R-derived paralogs of OMPs.
Stable knock-down of vomeronasal receptor genes in transgenic Xenopus tadpoles.


Olfactory marker protein (OMP) gene deletion causes altered physiological activity of olfactory sensory neurons.
Observations provide strong support for the conclusion that OMP is a novel modulatory component of the odor detection/signal transduction cascade and postsynaptic changes in gene expression induced by peripheral olfactory deafferentation or naris blockade are confirmed.
Olfactory marker protein mRNA is found in axons of olfactory receptor neurons
It is hypothesized that the immature nature of the cytoskeleton and, specifically, the lack of tightly bundled microtubules allows transport of particular mRNAs in olfactory receptor axons.
Human and rodent OMP genes: conservation of structural and regulatory motifs and cellular localization.
The OMP gene from human and mouse is cloned and conservation of gene structure, protein sequence, and Olf-1 and upstream binding region (UBE) regulatory domains are demonstrated, allowing additional insight into mechanisms that may participate in the stringent regulation of the expression of this neuronal-specific protein.
Olfactory marker protein gene: its structure and olfactory neuron-specific expression in transgenic mice.
Functional OMP regulatory sequences were demonstrated in transgenic mice and showed the absence of introns and the lack of CAAT and TATA boxes in the 5' flanking region.
Immunocytochemical identification of primary olfactory afferents in rainbow trout
It is likely that the organization of afferents in the trout olfactory bulb is similarly well regulated during normal receptor cell replacement.
Molecular Cloning of Olfactory-Specific Gene Products
The major thrust of this chapter is to consider the use of biochemical and molecular biological approaches in the study of the regulation of specific gene expression in the peripheral olfactory system.
Olfactory neuron-specific protein is translated from a large poly(A)+ mRNA.
In vitro synthesized OMP is synthesized directly without the intermediate formation of a larger polypeptide precursor, indicating that OMP mRNA contains untranslated regions that are four to five times larger than the coding region.