Two non-complementing genes encoding enzymatically active methylenetetrahydrofolate reductases control methionine requirement in fission yeast Schizosaccharomyces pombe.

@article{Naula2002TwoNG,
  title={Two non-complementing genes encoding enzymatically active methylenetetrahydrofolate reductases control methionine requirement in fission yeast Schizosaccharomyces pombe.},
  author={Nicolas Naula and Claudia Walther and Dominique Baumann and Martin Ernst Schweingruber},
  journal={Yeast},
  year={2002},
  volume={19 10},
  pages={841-8}
}
By transforming two methionine auxotrophic mutants from fission yeast Schizosaccharomyces pombe with a wild-type gene library, we defined two genes, met9 and met11, which both encode a methylenetetrahydrofolate reductase. The genes cannot complement each other. We detected single transcripts for both. In vitro measurements of enzymatic activities showed that the met11-encoded enzyme was responsible for only 15-20% of the total methylenetetrahydrofolate reductase activity. A strain in which gene… CONTINUE READING

Connections & Topics

Mentioned Connections BETA
Our results are in accordance with the assumption that the two methylenetetrahydrofolate reductases generate the methyl groups necessary for methionine synthetase to convert homocysteine to methionine , and suggest that expression of the two genes is an important parameter in the control of methionine biosynthesis .
Our results are in accordance with the assumption that the two methylenetetrahydrofolate reductases generate the methyl groups necessary for methionine synthetase to convert homocysteine to methionine , and suggest that expression of the two genes is an important parameter in the control of methionine biosynthesis .
All Topics