Two new grape cultivars, bud sports of Cabernet Sauvignon bearing pale-coloured berries, are the result of deletion of two regulatory genes of the berry colour locus

  title={Two new grape cultivars, bud sports of Cabernet Sauvignon bearing pale-coloured berries, are the result of deletion of two regulatory genes of the berry colour locus},
  author={Amanda R. Walker and Elizabeth Lee and Simon P. Robinson},
  journal={Plant Molecular Biology},
Bud sports are infrequent changes in phenotype affecting shoots of woody perennials but the molecular basis of these mutations has rarely been identified. In this report, we show that the bronze-coloured berries of the Malian cultivar, a documented bud sport of the wine grape Cabernet Sauvignon (Vitis vinifera L.), lack anthocyanins in the subepidermal cells compared to the red/black berried Cabernet Sauvignon in which both the epidermis and several subepidermal cell layers contain anthocyanin… 

Color Intensity of the Red-Fleshed Berry Phenotype of Vitis vinifera Teinturier Grapes Varies Due to a 408 bp Duplication in the Promoter of VvmybA1

Quantitative real time PCR and HPLC analysis of leaf and berry samples showed that the GCE repeat number strongly correlates with an increase of the expression of VvmybA1 itself and the VvUFGT gene regulated by it and the anthocyanin content.

Molecular Characterization of a Bud Sport of Pinot gris Bearing White Berries

The molecular characterization of a white color mutation in Pinot gris shows a triallelic profile at the locus based on genotyping with a microsatellite DNA marker, indicating that genetically different cell layers, L1 and L2, coexisted in the berries.

Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. ‘Moscatel Galego’)

It is proposed that the reduced activity of the flavonoid tri-hydroxylated sub-branch and decreased anthocyanin synthesis and modification and methylation and acylation are the potential causes for the mild red-skinned coloration in the pigmented revertant.

Parentage of Merlot and related winegrape cultivars of southwestern France: discovery of the missing link

The discovery of the key genetic role of a previously unknown cultivar in the origins of some significant cultivars reinforces the importance of deep exploration, before it is too late, to discover original genotypes which have not yet been collected or referenced.


Alleles of the VvMybA1 gene were first identified in native Russian grape varieties of Chardonnay, Sibirkovskii, Cabernet Cortis and Sypun cherny varieties and found that the alleles of colored varieties have a structure characteristic for varieties with a pronounced color of berries, while uncolored varieties apparently have a specific reason for the loss of color.

A group of grapevine MYBA transcription factors located in chromosome 14 control anthocyanin synthesis in vegetative organs with different specificities compared with the berry color locus

The involvement of additional anthocyanin regulators in grapevine is demonstrated and an evolutionary divergence between the two grape color loci for controlling additional targets of the flavonoid pathway is suggested.

Transcriptomic analysis of Citrus clementina mandarin fruits maturation reveals a MADS-box transcription factor that might be involved in the regulation of earliness

This work analyzes the evolution of the transcriptome during fruit ripening in 3 sport mutations derived from the Fina clementine (Citrus clementina) mandarin: Clemenules (CLE), Arrufatina (ARR) and Hernandina (HER) that differ in their harvesting periods to identify a transcription factor that could be involved in the early phenotype.



Association of VvmybA1 gene expression with anthocyanin production in grape (Vitis vinifera) skin-color mutants

By using white-skinned cultivars (‘Italia’ and ‘Muscat of Alexandria’) and putative red-skinned sports (‘Ruby Okuyama’ and ‘Flame Muscat’) from the white cultivars, we analyzed the expression and

Anthocyanin composition and anthocyanin pathway gene expression in grapevine sports differing in berry skin colour

The results suggest that control of 3‐monoglucoside synthesis and modification vary in the sports, and the lack of anthocyanins in white‐skinned varieties or sports correlates with a lack of expression of the UFGT gene, although the expression of some other flavonoid pathway genes was also decreased.

Diversification within grapevine cultivars goes through chimeric states.

The accumulation of somatic mutations and cell layer rearrangements allowed us to deduce the relationships between the various genotypes and to open a way for understanding the diversification process and the phylogeny in the 'Pinot' group.

Genetic structure and differentiation in cultivated grape, Vitis vinifera L.

The overall organization of genetic diversity suggests that the germplasm of cultivated grape represents a single complex gene pool and that its structure is determined by strong artificial selection and a vegetative mode of reproduction.

Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis

The results strongly suggest that MybA genes are involved in the regulation of anthocyanin biosynthesis in the grape via expression of the UFGT gene.

Genetic and physical mapping of the grapevine powdery mildew resistance gene, Run1, using a bacterial artificial chromosome library

A comparison of physical and genetic mapping data indicates that recombination is severely repressed in the vicinity of Run1, possibly due to divergent sequence contained within the introgressed fragment from M. rotundifolia that carries the Run1 gene.

Chimerism in grapevines: implications for cultivar identity, ancestry and genetic improvement

It appears that grapevine chimerism can not only modify phenotype but can also impact on grapevine improvement as both genetic transformation and conventional breeding strategies separate mutations in the L1 and L2 cell layers.

Association of dwarfism and floral induction with a grape ‘green revolution’ mutation

The conversion of tendrils to inflorescences in the mutant demonstrates that the grapevine tendril is a modified inflorescence inhibited from completing floral development by GAs, providing genetic evidence that GAs inhibit flowering in grapevine.

Analysis of flower pigmentation mutants generated by random transposon mutagenesis in Petunia hybrida.

Findings indicate that mutants for novel loci are most likely tagged by dTph1 elements opening the way for their isolation.

Flower color variation: a model for the experimental study of evolution.

  • M. CleggM. Durbin
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2000
The study of flower color polymorphisms in the morning glory is reviewed as a model for the analysis of adaptation and it is found that almost all of the mutations that determine phenotypic differences are the result of transposon insertions.