Two new fluorophore pairs, tyrosine-4'-aminophenylalanine and tyrosine-4'-dimethylaminophenylalanine, in the determination of molecular dimensions in peptides.


Peptides and polypeptides containing tyrosine and 4'-aminophenylalanine or tyrosine and 4'-dimethylaminophenylalanine were studied by electronic absorption and fluorescence spectroscopy. Positions of the band maxima, pKa values of the aromatic ammonium group and distances between the two fluorophores in seven different compounds are compared. 


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