Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor.

@article{Kamionka2004TwoMI,
  title={Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor.},
  author={Annette Kamionka and Joanna Bogdanska-Urbaniak and Oliver R. Scholz and Wolfgang Hillen},
  journal={Nucleic acids research},
  year={2004},
  volume={32 2},
  pages={842-7}
}
We report for the first time the in vitro characterization of a reverse tetracycline repressor (revTetR). The dimeric wild-type repressor (TetR) binds to tet operator tetO in the absence of the inducer anhydrotetracycline (atc) to confer tight repression. We have isolated the revTetR G96E L205S mutant, which, contrary to TetR, binds tetO only in the presence of atc. This reverse acting mutant was overproduced and purified. Effector and DNA binding properties were analyzed by EMSA and quantified… CONTINUE READING