Two monoclonal antibodies to actin: one muscle selective and one generally reactive.

@article{Lessard1988TwoMA,
  title={Two monoclonal antibodies to actin: one muscle selective and one generally reactive.},
  author={James L. Lessard},
  journal={Cell motility and the cytoskeleton},
  year={1988},
  volume={10 3},
  pages={349-62}
}
Two IgG1, kappa monoclonal antibodies (Mab) against actin have been obtained from a fusion in which chicken gizzard actin was used as the immunogen. One Mab, designated B4, shows a preferential reactivity toward enteric smooth muscle actin but also cross-reacts with skeletal, cardiac, and aorta actins on the basis of immunoblots, ELISA assays, and indirect immunofluorescence. However, this antibody does not react with either cytoplasmic actin in any of these assay systems. A second Mab… CONTINUE READING